［Objective］This study aims to mitigate the adverse effects caused by the adhesion ofmarine fouling organisms to the windows of optical instruments during their service in the marineenvironment.［Methods］ Using 3，3，3-trifluoropropylmethyldimethoxysilane（MTFPS） as the monomer，a liquid-like molecular brush slippery surface which featuring a main chain composed ofsiloxane bonds was prepared via covalent grafting on the surface of glass substrate through acid-catalyzed polycondensation. The structure and morphology of the molecular brush slippery surface werecharacterized systematically. Additionally，key performances of the slippery surface were investigated， including liquid repellency，light transmittance，stability，and anti-fouling efficacy against proteins， bacteria，and algae.［Results］The liquid-like molecular brush slippery surface formed by chemicalbonding exhibits excellent liquid repellency，which can effectively prevent liquid from wetting thesurface and maintain high light transmittance. Furthermore，the surface-bonded liquid-like molecularbrush demonstrates superior anti-adhesion performance against bovine serum albumin，Escherichia coli，Staphylococcus aureus，Chlorella pyrenoidosa and Phaeodactylum tricornutum.［Conclusion］This work provides novel insights and strategies for the development of anti-fouling technologies tailored tothe windows of optical instruments in the marine field.