【Objective】To explore the application potential of bio-based materials in the field ofwater-based polyurethanes and reduce reliance on petroleum-based products.【Methods】Using poly（trimethylene ether）glycol（PO3G），polycarbonate diol（PCDL），dimethylolpropionic acid（DMPA）， and isophorone diisocyanate（IPDI） as the main raw materials，a series of bio-based water-based polyurethane dispersions with a solid content of 50% were prepared，and the printing performance wastested by preparing them into printing pastes. Characterization analysis of the product was conductedusing infrared spectroscopy，and the effects of PCDL dosage on the stability of dispersion，thermodynamic properties，mechanical properties，water contact angle，and printing performance of the film were discussed.【Results】With the increase of PCDL content，the elongation at break of the film decreased，while the strength and hardness gradually improved. When the mass of PCDL and PO3G was40 g and 80 g respectively，the water contact angle on the coating surface was increased to 83. 9°. The XRDtesting results indicated that the material was an amorphous polymer. Additionally，in printing tests，the soaping fastness of the cloth board was significantly improved.【Conclusion】This study demonstrates the feasibility of replacing petroleum-based products with bio-based alternatives，providing theoretical support for the application of bio-based water-based polyurethanes in the printing field.