［Objective］To evaluate the mold resistnce and the inhibitory effects and mechanismsof nano-ceramic coatings against enteric viruses and the Omicron BA. 5 variant of coronavirus.［Methods］A sterilized metal plate with no antiviral effect was used as a substrate，the long-lastingself-sterilizing nano-ceramic coating was prepared by using the polysiloxane aqua-based technologyand nano room-temperature ceramization technology. The properties of mold resistnce，anti-enterovirus（EV71）， and anti-coronavirus（Omicron BA. 5）of the coating and its aged version were tested using 8 types of mold and 2 viruses.［Results］ The nano-ceramic coating demonstrated excellent mold resistnce，with the mold resistnce grade of 0（no mold growth on the test sample），and it had significantinhibitory effect against enterovirus and coronavirus with the inhibition rate >99%. Even after aging，the coating still showed an inhibition rate of >99% against enterovirus 71 and coronavirus. Immunofluorescence assays and quantitative fluorescence PCR indicated that the nano-ceramic coatingreduced the expression of C4a genes and VP1 proteins，thereby inhibiting enterovirus from adsorption and penetration into host’s cells. It also lowered the expression of S，E，and N genes and S and NP proteins，damaging the coronavirus surface structure，disrupting its binding to host’s cells，and blocking its replication within host’s cells.［Conclusion］The long-lasting antibacterial and antiviral nano-ceramic coating exhibited significant mold resistnce and antiviral properties against enteroviruses andcoronavirus. It provides a long-lasting，broad-spectrum，and eco-friendly antibacterial and antiviral coating solution，offering a new approach for surface treatment in the high-risk infection scenarios andupgrading coatings from passive protection to active intervention.