In this paper，the Schiff base and its copper complex were synthesized usingbiologically active indole-3-fromaldehyde and aminothiourea. The structure of the prepared Schiff base and the coordinated mode of the copper（II）complex were identified by ¹H NMR， FT-IR and UV spectroscopy. The antibacterial properties of products were testd usingStaphylococcus aureus（S. aureus）and Escherichia coli（E. coli）. The results showed that the prepared copper（II）complex had a good antibacterial effect on both gram-positive and gram-negative bacteria，and had a minimum inhibitory concentration of 0. 40 g/L and 0. 95 g/L for S. aureus and E. coli respectively，demonstrating broad-spectrum antimicrobial properties. Thecoating using indole methyl thiosemicarbazone copper（II）complex as antibacterial agent also showed excellent antibacterial performance，which could kill the attached E. coli on the coatingsurface when 5% of copper complex was contained. Based on bacterial morphology observation by electron microscope，it could be speculated that the copper（II）complex killed bacteria bydirectly acting on the cell membrane，showing good antibacterial performance.