The multiple self-crosslinking waterborne acrylic resin dispersion wasprepared by solution polymerization through epoxy ester and vinyl monomer. The used epoxyester was obtained by the reaction of epoxy resin（EP-20）with unsaturated fatty acid and N-methoxy methacrylamide（NMMA）. It was systematically studied for the comprehensiveproperties of the resin film that was influenced by the proportion of epoxy ester and methacrylicacid，crosslinking monomer NMMA，acetoacetoxyethyl methacrylate（AAEM），β -gelatin protein，unsaturated fatty acid or preparation process. The results showed that multiple self-crosslinking waterborne acrylic coatings indicated excellent comprehensive properties，the surface drying time was 28 min，the water resistance was 14 d，the hardness and adhesion were 3H and 0 grade，respectively，and it also exhibited good solvent resistance.