选用具有生物活性的吲哚-3-甲醛与氨基硫脲制备了席夫碱及其铜（II）配合物，通过核磁共振氢谱、红外及紫外光谱等手段鉴定了其结构并确认配合物的配位方式。选用金黄色葡萄球菌（S. aureus）与大肠埃希氏菌（E. coli）测试了席夫碱及其铜（II）配合物的抗菌性。结果表明：所制备的铜（II）配合物对革兰氏阳性与革兰氏阴性菌均具有较好的抗菌效果，对金黄色葡萄球菌（S. aureus）和大肠埃希氏菌（E. coli）的最小抗菌浓度分别为 0. 40 g/L和 0. 95 g/L，具备广谱抗菌性。以吲哚甲醛氨基硫脲席夫碱铜（II）配合物作为抗菌剂制备的抗菌涂层同样具有良好的抗菌效果，质量分数为 5%时就能够杀灭抗菌涂层上黏附的大肠埃希氏菌（E. coli）。通过电镜观察细菌形貌推测吲哚甲醛氨基硫脲席夫碱铜（II）配合物可能通过直接作用于细胞膜将其杀灭，具有较优的抗菌性。

In this paper，the Schiff base and its copper complex were synthesized usingbiologically active indole-3-fromaldehyde and aminothiourea. The structure of the prepared Schiff base and the coordinated mode of the copper（II）complex were identified by ¹H NMR， FT-IR and UV spectroscopy. The antibacterial properties of products were testd usingStaphylococcus aureus（S. aureus）and Escherichia coli（E. coli）. The results showed that the prepared copper（II）complex had a good antibacterial effect on both gram-positive and gram-negative bacteria，and had a minimum inhibitory concentration of 0. 40 g/L and 0. 95 g/L for S. aureus and E. coli respectively，demonstrating broad-spectrum antimicrobial properties. Thecoating using indole methyl thiosemicarbazone copper（II）complex as antibacterial agent also showed excellent antibacterial performance，which could kill the attached E. coli on the coatingsurface when 5% of copper complex was contained. Based on bacterial morphology observation by electron microscope，it could be speculated that the copper（II）complex killed bacteria bydirectly acting on the cell membrane，showing good antibacterial performance.